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Bfgf is a multifunction nutritional cell factor, it is firstdiscovered that bfgf has the mitosis ac

Bfgf (FGF-2) is a multifunction nutritional cell factor, it isfirst discovered that bfgf has the mitosis activating effects withcultured fibroblast, later it is discovered that bfgf also hasmitosis activating effects on many other cells, especially tomesoderm and neuroectoderm derived cells. Besides the mitosisactivating effects, bfgf also has many other biological functions,such as inducing cell to change chemotaxis property, inducing orinhibiting the expression of certain cell type specific proteins,regulating cell differentiation, also involved in the functioningof endocrine system and neural system. Recombinant human fgf iscomposed with 155 amino acids, the human rh-bfgf coding cdna wasobtained from peripheral blood leukocytes mrna, its cdna wassynthesized by reverse-transcription, and it was subcloned intoexpression vector and expressed in e. Coli dh5a. The recombinanthuman bfgf is purified by our unique biochemical purificationmethod. Purity: > 95% , as determined by sds-page analysis.Molecular weight: 17. 0 -10% kda Activity: the rh-bfgf is fullybiologically active compared with certain standards. The ed (50)was determined by the dose-dependent stimulation of thymidineuptake by balb / c 3t3 cells (pnas 94, 10205, 1997) is < 1 ng /ml, corresponding to a specific activity of > 1x10 (e6) units /mg. Formulation: lyophilized from a 0. 22mm filtered solution in 20mm PB in absence of any carrier protein.