Advantage

1.Certificate:ISO13485:2003, GMP
2.Sensitivity>9/10
3.Specificity>14/15
4.Precision: CV<15%

INTEND USED

Anti-HIV1 2 is an enzyme-linked immunosorbent assay (ELISA) forthe qualitative determination of antibodies to humanimmunodeficiency virus type 1 and/or 2 in human serum orplasma.

DAAN HBsAg ELISA is an enzyme-linked immunosorbent assay (ELISA)for the qualitative determination of Hepatitis B SurfaceAntigen(HBsAg) in human serum or plasma.

Sensitive enzyme immunoassays for the detection of HBsAg werefirst described by Engvall and Perlmann and VanWeemen and Schuursin 1971. In 1976 and 1977, solid phase “sandwich” enzymeimmunoassays were developed in which HBsAg was captured on a solidphase coated with polyclonal antibodies against HBsAg (anti-HBs)and then detected with anti-HBs conjugated to an enzyme. In recentyears, monoclonal anti-HBs assays have been developed for thedetection of HBsAg.

The wells of the polystyrene microplate strips are coated withmouse monoclonal anti-HBs. Both human serum or plasma andpolyclonal anti-HBs labeled with horseradish peroxidase areincubated in these coated wells. HBsAg, if present, will bind tothe solid phase antibody and the labeled antibody resulting in theHBsAg being sandwiched between the solid phase antibody and labeledantibody. Excess unbound HBsAg and labeled antibodies are removedby washing. Substrate solution containing hydrogen peroxide and3,3’,5,5’-tetramethyl- benzidine(TMB) is then added to each well.The presence of HBsAg is indicated by the presence of a blue colorafter substrate addition. Reaction is terminated by addition ofsulphuric acid. The intensity of the color is measuredspectrophotometrically at 450nm and is proportional to the amountof HBsAg present in the specimen.

1. Microplate  1 plate (96 wells). 8 stripsper plate, each with 12 wells coated with anti-HBs.
2. Positive Control  1 vial (1.0ml).Inactivated Human serum containing HBsAg. Preservative: Bronidox(2ml/L).
3. Negative Control  1 vial (1.0ml).Inactivated Human serum without HBsAg. Preservative: Bronidox(2ml/L).
4. Conjugate  1 vial (6ml).  Thesolution contains HRP-labeled anti-HBs, with PBS(0.01mol/L) andstabilizing proteins, ready to use.
5. 5.       Sample Diluent  1 vial (3.5ml). 

Contains stabilizing proteins and detergent, Preservative:Bronidox (2ml/L).

1. Wash Buffer(25´)  1 vial(20ml).  Diluted 25-fold in distilled water as described insection of Preparation of Reagent.
2. Substrate Solution A  1 vial (6ml). Hydrogen peroxide-urea (approx. 0.6g/L) in citrate-acetate buffersolution (10 mmol/L).
3. Substrate Solution B  1 vial (6ml). Tetramethyl benzidine dihydrochloride (10g/L).
4. Stop Solution  1 vial (6ml).  2N sulphuric acid.
5. Plate Covers  2 pieces
6. Instruction manual  1 copy